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KMID : 0545119930030030194
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Journal of Microbiology and Biotechnology
1993 Volume.3 No. 3 p.194 ~ p.198
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The Preparation of Crystalline Mannobiose from Brown Copra Meal Using the Enzyme System and Yeast Fermentation
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Park Gwi-Gun
Chang Hak-Gil
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Abstract
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β-1,4-Mannobiose was prepared by the enzymatic hydrolysis of brown copra meal and the subsequent elimination of mono-saccharides from the resultant hydrolysate with a yeast. The enzyme system hydrolyzed brown copra meal and produced monosaccharides and β-1,4-mannobiose without other oligomers at the final stage of the reaction. Brown copra meal (30g) was hydrolyzed at 50℃ and pH 5 for 48 hr with the crude enzyme solution (300㎖) from Penicillium purpurogenum. By the elimination of monosaccharides from the hydrolysis products with a yeast (Candida parapsilosis var. komabaensis k-75), 5.2g of crystalline mannobiose was obtained without the use of chromatographic techniques. After 50 hours of yeast cultivation, the total sugar content fell from 3.5% to 2.4%, and the average degree of polymerization rose from 1.8 to 2.2.
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